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The fungal cell wall as a target for antifungal therapies Partner 4
Département de
Génie Biochimique,
Principal
scientist
Other
scientists 135, Avenue de Rangeuil
Experience of the participating organisation and scientists Participating organisation The proposed work will be carried out by the group of Molecular Physiology of Lower Eukaryotes and the Biochips platform of the Toulouse Genopole directed by Prof. Jean François. The Molecular Physiology team is part of the Biotechnology and Bioprocess (Unité Mixte CNRS and INRA) which is housed in the National School of Applied Sciences, Toulouse (France). This Research Unit is fully equipped with modern analytical, biochemical, molecular biology and fermentation tools. The team of Prof. François has a wide expertise in yeast physiology, including functional genomics, genetic and metabolic regulation of central metabolic and signal transduction pathways in yeast and filamentous fungi. The Biochips platform, which is housed in the INSA Campus, belongs to the Toulouse Genopole, one of the eight elected Genopole in France. This platform is fully equipped with all material facilities for transcriptomics and proteomics analyses (spotter for DNA arrays and protein chips, automate for hybridization, laser scanner, phosphofluoroimager, microimager, Biacore 3000, Atomic Force Microscopy, Scanning Electron microscopy) as well as biomathematic and bioinformatic tools and expertise for data analysis, and with trained scientists in this post-genomic research activity. Participating scientists
Prof. Jean François,
the leader of the Group, has more than
15 years expertise in the field of
Yeast Physiology. Moreover, he
recently coordinated an EU program in
the 5th FP called "Eurocellwall" which
involved 8 laboratories, one SME and a
big-pharma from 6 different EU
countries, and whose purpose it was to
exploit the molecular knowledge of the
yeast Saccharomyces cerevisiae cell wall with the
aim to develop high throughput
screening of antimicrobial agents. A
major conclusion of this program was
to point out the major role of genes
coding for remodeling enzymes in the
cell wall organization, and hence to
provide decisive arguments for these
genes as putative antifungal targets.
Prof. François is also the head of the
Biochips platform of the Toulouse
Genopole. He is author and co-author
of more than 60 peer-reviewed papers.
Dr Véronique Leberre is Research
Associate at CNRS, and the responsible
scientist of the Biochips platform.
She is a Biochemist, specialized in
DNA and protein array fabrication and
analysis, and has also expertise in
protein-ligand interaction using
Biacore. Relevant publications Martin-Yken, H., Dagkessamanskaia, A., Basmaji, F., Lagorce, A., François, J. (2003) The interaction of Slt2 MAP kinase with Knr4 is necessary for signalling through the cell wall integrity pathway in Saccharomyces cerevisiae. Mol. Microbiol. 49:23-35. Lagorce, A., Hauser, N., Labourdette, D., Rodriguez, C., Martin-Yken, H., Arroyo, J., Hoheisel, J., François, J. (2003) Genome wide analysis in response to cell wall mutations of Saccharomyces cerevisiae. J. Biol. Chem. 278:20345-20357. Le Berre V, Trévisiol E, Dagkessamanskaia A, Caminade AM, Majoral JP, Meunier B, François J. (2003) Dendrimeric coating of glass slides for sensitive DNA microarrays. Nucleic Acid Res 31, 88:1-8. François, J., Parrou, J.L (2001) Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol. Rev. 25:125-145. Trévisiol, E., Renard, A., Defrancq, E., Lhomme, J. (2000) Fluorescent labelling of oligodeoxyribonucleotides by the oxyamino-aldehyde coupling reaction. Nucleosides, Nucleotides & Nucleic Acids, 19:1427-1439.
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